carbon 14 labeled tissue

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From:GSennello@nexstar.com
To:HistoNet@pathology.swmed.edu
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Hello all,
I have been asked to do auto-radiography on 14 C labeled rat tissue.  The
animals are receiving a single oral dose of
 250 uCi/kg labeled drug that will probably not be absorbed well (we are
thinking it will end up it the gut).  I will be looking at kidney, liver
and bone.  My questions are:  (1) will the processor,  the solutions and
the waste sections  be hot at this level?  In the past someone here dosed
at 1/5 this level and it was not detectable.  (2) should I try film vs.
emulsion, in the past nothing was seen with emulsion after  30 and 60 day
exposures.

Thanks    Gina Sennello


PS. I will soon be doing WBA (whole body autoradiography), we are waiting
for the macro-cryostat to arrive and I'm sure I will have many questions.
Is there anyone out there who would be willing to let me call them?




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