carbon 14 labeled tissue
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From: | GSennello@nexstar.com |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hello all,
I have been asked to do auto-radiography on 14 C labeled rat tissue. The
animals are receiving a single oral dose of
250 uCi/kg labeled drug that will probably not be absorbed well (we are
thinking it will end up it the gut). I will be looking at kidney, liver
and bone. My questions are: (1) will the processor, the solutions and
the waste sections be hot at this level? In the past someone here dosed
at 1/5 this level and it was not detectable. (2) should I try film vs.
emulsion, in the past nothing was seen with emulsion after 30 and 60 day
exposures.
Thanks Gina Sennello
PS. I will soon be doing WBA (whole body autoradiography), we are waiting
for the macro-cryostat to arrive and I'm sure I will have many questions.
Is there anyone out there who would be willing to let me call them?
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