Re: carbon 14 labeled tissue
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From: | Abizar Lakdawalla <abizarl@innogenex.com> |
To: | GSennello@nexstar.com |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
hi gina, there is an increasing trend to detect a drug compound in vivo by
immunohistochemical means. This of course presupposes that you have generated
a polyclonal antibody to the drug.
Abizar
www.innogenex.com
GSennello@nexstar.com wrote:
> Hello all,
> I have been asked to do auto-radiography on 14 C labeled rat tissue. The
> animals are receiving a single oral dose of
> 250 uCi/kg labeled drug that will probably not be absorbed well (we are
> thinking it will end up it the gut). I will be looking at kidney, liver
> and bone. My questions are: (1) will the processor, the solutions and
> the waste sections be hot at this level? In the past someone here dosed
> at 1/5 this level and it was not detectable. (2) should I try film vs.
> emulsion, in the past nothing was seen with emulsion after 30 and 60 day
> exposures.
>
> Thanks Gina Sennello
>
> PS. I will soon be doing WBA (whole body autoradiography), we are waiting
> for the macro-cryostat to arrive and I'm sure I will have many questions.
> Is there anyone out there who would be willing to let me call them?
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