Re: Spermatozoa stains (New and other fuchsines)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Roberta Horner <rjr6@psu.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Thu, 3 Aug 2000, Roberta Horner wrote:
> Does anyone have a procedure for a stain for spermatozoa? Or could someone
> tell me if new fuchsin is also known by another name? I have Berg's method
> but it uses new fuchsin. I need this as soon as possible for a ram sale.
New fuchsine is one of the dyes that compose basic fuchsine. The
others are pararosaniline, rosaniline and magenta II. Of these,
only pararosaniline and new fuchsine are commercially available
as single "pure" dyes.
For most purposes it doesn't matter if you use one of the pure
dyes or a mixture. For the aldehyde-fuchsine stain you must use
pararosaniline or a basic fuchsine that is mostly pararosaniline
(Mowry & Emmel 1977 J Histochem Cytochem 25:239). The only other
methods I know about for which a single dye is advisable (possibly
necessary) are those for which you make a diazonium salt for use
in esterase or peptidase enzyme activity histochemistry (hexazonium
pararosaniline or hexa-azotized new fuchsine).
Both basic fuchsine and pararosaniline are available as certified
dyes. New fuchsine (CI 42520) is not certified as such. A dye
certified as basic fuchsine could contain only pararosaniline or
new fuchsine, or it could be a mixture of 2, 3 or all 4 dyes (often
with a high percentage of rosaniline). Rosaniline (C.I. 42510) and
magenta II (has no C.I. number) can be made only by separation
from mixtures, which isn't commercially worthwhile. They are not
sold as single dyes because there would be no known use for them.
In this email I have used -ine rather than -in endings for the
names of the dyes because this is the chemically correct spelling
in English, and is used worldwide in the literature of dyes.
Most catalogues still use fuchsin, pararosanilin etc., following
an older (50 yrs +) tradition in which these dyes were derivatives
of the German "Anilin" rather than the English/French/US "aniline."
Amines have ended in -ine for a century or more in the English
tradition.
The -in ending is used for substances that are _not_ derived from
organic bases (amines). Eosin, erythrosin and haematoxylin are
good examples. So are dextrin and insulin.
Sorry if this doesn't tell you how to stain spermatozoa. Someone
else is sure to do that. This should put you & others in the
picture about the various basic fuchsine dyes.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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