Re: Spermatozoa stains
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From: | Lee & Peggy Wenk <lpwenk@mail.netquest.com> |
To: | Roberta Horner <rjr6@psu.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
You could probably substitute Basic Fuchsin = pararosanilin
(CI 42500) for the New Fuchsin (CI 42520).
They are from the same family of dyes, and have the same
general formula/chemical shape, but New Fuchsin has
3 additional methyl groups (-CH3). This gives the
New Fuchsin a slightly deeper shade than Basic Fuchsin.
I've used either to make Schiff, and they both work.
If I remember correctly, the Berg stain is a carbol-fuchsin
stain with a methylene blue counterstain (similar to Kinyoun,
but with a lower percent of dye, and differentiated with
acetic acid). Since Kinyoun uses Basic Fuchsin, and the
Berg uses New Fuchsin, and both are carbol-fuchsin, it
seems like you should be able to substitute.
Because Basic Fuchsin is a lighter shade than New Fuchsin,
the end color might be a lighter shade if you substituted
the Basic Fuchsin for the New Fuchsin. You would probably
only notice this difference if you ran the two slides
side by side and did a comparison.
On the other hand, Giemsa and cytology Pap stains also
stain spermatozoa. Has anyone tried the Diff-Quik for
this?
Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073
Roberta Horner wrote:
>
> Does anyone have a procedure for a stain for spermatozoa? Or could someone
> tell me if new fuchsin is also known by another name? I have Berg's method
> but it uses new fuchsin. I need this as soon as possible for a ram sale.
> Thank you very much.
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University
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