Re: Spermatozoa stains

<< Previous Message | Next Message >>
From:Lee & Peggy Wenk <>
To:Roberta Horner <>
Content-Type:text/plain; charset=us-ascii

You could probably substitute Basic Fuchsin = pararosanilin 
(CI 42500) for the New Fuchsin (CI 42520).

They are from the same family of dyes, and have the same 
general formula/chemical shape, but New Fuchsin has 
3 additional methyl groups (-CH3). This gives the 
New Fuchsin a slightly deeper shade than Basic Fuchsin.

I've used either to make Schiff, and they both work.

If I remember correctly, the Berg stain is a carbol-fuchsin
stain with a methylene blue counterstain (similar to Kinyoun,
but with a lower percent of dye, and differentiated with
acetic acid). Since Kinyoun uses Basic Fuchsin, and the
Berg uses New Fuchsin, and both are carbol-fuchsin, it 
seems like you should be able to substitute.

Because Basic Fuchsin is a lighter shade than New Fuchsin,
the end color might be a lighter shade if you substituted
the Basic Fuchsin for the New Fuchsin. You would probably 
only notice this difference if you ran the two slides
side by side and did a comparison.

On the other hand, Giemsa and cytology Pap stains also
stain spermatozoa. Has anyone tried the Diff-Quik for

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073

Roberta Horner wrote:
> Does anyone have a procedure for a stain for spermatozoa?  Or could someone
> tell me if new fuchsin is also known by another name? I  have Berg's method
> but it uses new fuchsin.  I need this as soon as possible for a ram sale.
> Thank you very much.
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University

<< Previous Message | Next Message >>