Re: Hepatitis C immunos

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From:Kappeler Andreas <kappeler@patho.unibe.ch>
To:Histonet <HistoNet@pathology.swmed.edu>
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Hi Margaret, hi Alexander

Same experience here (FFPE material): Novocastra's mo-a-HCV, clone MMM33,
stained about the same proportion of known HCV+ as of control cases ...
Similar results with clone HCV88 (BioGenex), no positive result with clones
N57 (HCV NS-3 protein) and C39 (HCV core protein) (both from Biodesign).
Never tried clone Tordji-22 as a colleague of mine told me - after extensive
testing - that he had never had any success with this antibody.
So if anybody can come forward with an antibody and a protocol that reliable
identifies HCV+ patients (and only these ...) on FFPE - please do not
hesitate to share the information with the Histonet community. Thanks!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

P.S. Alexander, hope you had a safe trip back from Cambridge!




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