Re: Dominici's Stain

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From:"J. A. Kiernan" <>
To:"Ryan.Linda" <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Wed, 9 Aug 2000, Ryan.Linda wrote:

> Anybody with experience with Dominici's stain?  I would appreciate the
> staining procedure and/or any information about this old stain.

I tried it once a long time ago. I can't remember the details,
but wasn't impressed enough to try it again. A slightly different
method was given by M.Gabe in his "Histological Techniques" (1976),
with some discussion of fixation and what to look for while

Dominici's triple stain.  Compt rend. Soc. Biol. 54:221 (1902)
(Cited from P.Gray 1954 The Microtomist's Formulary and guide, p.351)

Solution A.
Water 100 ml
Eosin Y 0.5 g
Orange G 0.5 g

Solution B.
Toluidine blue 0.5 g
Water 100 ml

Paraffin sections to water.
Stain in A for 5-10 min.
Quick rinse in water.
Stain in B for 20-30 sec.
Quick rinse in water.
95% alcohol "until differentiated"
Complete the dehydration, clear in xylene and cover.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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