On Wed, 9 Aug 2000, Ryan.Linda wrote:

> Anybody with experience with Dominici's stain?  I would appreciate the
> staining procedure and/or any information about this old stain.

I tried it once a long time ago. I can't remember the details,
but wasn't impressed enough to try it again. A slightly different
method was given by M.Gabe in his "Histological Techniques" (1976),
with some discussion of fixation and what to look for while
differentiating. 
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Dominici's triple stain.  Compt rend. Soc. Biol. 54:221 (1902)
(Cited from P.Gray 1954 The Microtomist's Formulary and guide, p.351)

Solution A.
Water 100 ml
Eosin Y 0.5 g
Orange G 0.5 g

Solution B.
Toluidine blue 0.5 g
Water 100 ml

Paraffin sections to water.
Stain in A for 5-10 min.
Quick rinse in water.
Stain in B for 20-30 sec.
Quick rinse in water.
95% alcohol "until differentiated"
Complete the dehydration, clear in xylene and cover.
________________________________________________


 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1