Re: Daily Digest

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From:Judy Trogadis <>
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> Does anyone have a good mouse eye processing protocol
> they could share?

 This is one we have used with success:

We remove the eyes, nick the inner edge of the sclera with a fine knife
to allow penetration of the fixative as well as to provide a landmark
orientation. The entire eyes are immersed in 4% paraformaldehyde
in PBS for 30 min. We then remove the sclera and lens and the eyes
are fixed for an additional 2 hours. Following a thorough PBS rinse,
the eyes are placed in a protective 30% sucrose solution in PBS,
allowed to sink to the bottom of the container and immediately
embedded in OCT. We then store the embedded tissue at -80deg
and cryosection as needed.

Judy Trogadis
Vision Science Research Program
Toronto Western Research Institute
399 Bathurst St.
Toronto, ON M5T 2S8
ph: 416-603-5088
fax: 416-603-5126

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