Peter,

To restrict the staining of dyes like Ethyl green to only the nucleus.
Apply them from an appropriate buffer at the optimum pH. We use 1% w/v Ethyl
green(or chloroform washed Methyl green) in Walpole acetate buffer pH 3.8.

Bryan

> ----------
> From: 	Peter Poon[SMTP:peterp@cafe.berkeley.edu]
> Sent: 	August 9, 2000 11:50 AM
> To: 	histonet
> Subject: 	methyl-green staining or ethyl-green staining
> 
> Oka.  we are currently trying to use ethyl-green as a nuclear counter
> stain to a immunohistostain for Estrogen receptor.  Most of the slides we
> do the background (neuropil) shows ups as pale blue in contrast to the
> nucleus, which is darker blue, but there are a few random slide in which
> the ethyl-green looks perfect, it stains the nucleus, but leaves the
> background unstained.  Does anyone know what happened? And how this can be
> duplicated. I'm at my wit's end on this one.
> 
> thanks,
> Peter Poon, histology rookie.
> 
> 
>