RE: Spermatozoa stains (New and other fuchsines)
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From: | "Nader, Alexander" <alexander.nader@wgkk.sozvers.at> |
To: | "'J. A. Kiernan'" <jkiernan@julian.uwo.ca> |
Reply-To: | |
Content-Type: | text/plain |
I remember when I was a student we used a nigrosin(e)-eosin method. maybe
nigrosin(e) is better known as acid black 2.
I found some information about this stain in
http://www.fertipro.com/products/inserts/invital.htm
But I thaught that nigrosin(e) is not a basic, but a acidic dye.
> -----Original Message-----
> From: J. A. Kiernan [mailto:jkiernan@julian.uwo.ca]
> Sent: Saturday, August 05, 2000 7:25 AM
> To: Roberta Horner
> Cc: Histonet
> Subject: Re: Spermatozoa stains (New and other fuchsines)
>
>
> On Thu, 3 Aug 2000, Roberta Horner wrote:
>
> > Does anyone have a procedure for a stain for spermatozoa?
> Or could someone
> > tell me if new fuchsin is also known by another name? I
> have Berg's method
> > but it uses new fuchsin. I need this as soon as possible
> for a ram sale.
>
> New fuchsine is one of the dyes that compose basic fuchsine. The
> others are pararosaniline, rosaniline and magenta II. Of these,
> only pararosaniline and new fuchsine are commercially available
> as single "pure" dyes.
>
> For most purposes it doesn't matter if you use one of the pure
> dyes or a mixture. For the aldehyde-fuchsine stain you must use
> pararosaniline or a basic fuchsine that is mostly pararosaniline
> (Mowry & Emmel 1977 J Histochem Cytochem 25:239). The only other
> methods I know about for which a single dye is advisable (possibly
> necessary) are those for which you make a diazonium salt for use
> in esterase or peptidase enzyme activity histochemistry (hexazonium
> pararosaniline or hexa-azotized new fuchsine).
>
> Both basic fuchsine and pararosaniline are available as certified
> dyes. New fuchsine (CI 42520) is not certified as such. A dye
> certified as basic fuchsine could contain only pararosaniline or
> new fuchsine, or it could be a mixture of 2, 3 or all 4 dyes (often
> with a high percentage of rosaniline). Rosaniline (C.I. 42510) and
> magenta II (has no C.I. number) can be made only by separation
> from mixtures, which isn't commercially worthwhile. They are not
> sold as single dyes because there would be no known use for them.
>
> In this email I have used -ine rather than -in endings for the
> names of the dyes because this is the chemically correct spelling
> in English, and is used worldwide in the literature of dyes.
> Most catalogues still use fuchsin, pararosanilin etc., following
> an older (50 yrs +) tradition in which these dyes were derivatives
> of the German "Anilin" rather than the English/French/US "aniline."
> Amines have ended in -ine for a century or more in the English
> tradition.
>
> The -in ending is used for substances that are _not_ derived from
> organic bases (amines). Eosin, erythrosin and haematoxylin are
> good examples. So are dextrin and insulin.
>
> Sorry if this doesn't tell you how to stain spermatozoa. Someone
> else is sure to do that. This should put you & others in the
> picture about the various basic fuchsine dyes.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
>
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