Hi Anette,

I don't know the specific answer to your questions, but have you checked 
Serotec? They seem to have the biggest selection of antibodies against a 
wide variety of species.

Cindy Farman
Sierra Biomedical
Sparks, NV

-----Original Message-----
From:	Anette Milton [SMTP:anette@biomeklab.aau.dk]
Sent:	Wednesday, August 09, 2000 4:40 AM
To:	HistoNet Server
Subject:	Re: Immun-histochemistry on dogs for pro-inflammatory cytokines

Hi
I will try again
Anette milton
----- Original Message -----
From: Anette Milton <anette@biomeklab.aau.dk>
To: HistoNet Server <histonet@pathology.swmed.edu>
Sent: Friday, August 04, 2000 12:07 PM
Subject: Immun-histochemistry on dogs for pro-inflammatory cytokines


Hi!
We are working on a model for wear debris aseptic implant loosening in 
dogs. Therefore we want establish a method for immunohistochemical staining 
for IL-1(alfa), IL-6 and TNF alfa on hard tissue. We embed the bone in cold 
MMA. Does anyone know antibodies for these cytokines with cross-reactivity 
for canine. Futhermore we would like to have antibodies for receptor CD 11b 
and vitronectin (osteoclasts)?
Best regards
Anette Milton
Orthopaedic Research Lab
Aarhus, Denmark