RE: Hepatitis immunos HELP desperate

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From:Louise Taylor <>
To:histonet <>
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What about using a non-biotin related secondary - such as DAKO's envsion? We
use this on our HepB and it works like a charm.

Louise taylor

-----Original Message-----
From: Jennings, Margaret A. []
Sent: 03 August 2000 09:35
To: HistoNet Server
Subject: Hepatitis immunos HELP desperate

I am working with HepC liver cells and trying to do immunocytochemistry. I
need help in blocking background in these cells. I have used normal serum,
peroxoblock, BSA, reagent A from Zymed's PAP kit (which is the recommended
kit for HCV. All of these in a variety of combinations. The 4%
paraformaldehyde fixed cells are false positive throughout. The MEOH fixed
cells are mostly stained around the periphery. IS ANYONE DOING HCV STAINING

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