Masson trichrome on frozens

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From:Sally Prouty <>
To:"Histonet Group (E-mail)" <>
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Does anyone have experience looking at masson trichrome's on frozens-. I am
used to looking at paraffin embedded tissue -specifically heart biopsies. I
am noticing a color present besides the red and green I am used to looking
at. Some tissue are staining a  fuschia like color. On some fibers, on
muscle specifically, the center of the fiber is almost clear instead of red
like it should be. Any suggestions. I did fix in 10%NBF before I postfixed
in Bouins. 

Sally Prouty B.S.,HTL
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52242

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