Immun-histochemistry on dogs for pro-inflammatory cytokines

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From:Anette Milton <>
To:HistoNet Server <>
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We are working on a model for wear debris aseptic implant loosening in dogs. Therefore we want establish a method for immunohistochemical staining for IL-1(alfa), IL-6 and TNF alfa on hard tissue. We embed the bone in cold MMA. Does anyone know antibodies for these cytokines with cross-reactivity for canine. Futhermore we would like to have antibodies for receptor CD 11b and vitronectin (osteoclasts)?
Best regards
Anette Milton
Orthopaedic Research Lab
Aarhus, Denmark 

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