Hepatitis immunos HELP desperate

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From:"Jennings, Margaret A." <jennings@mayo.edu>
To:HistoNet Server <HistoNet@Pathology.swmed.edu>
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I am working with HepC liver cells and trying to do immunocytochemistry. I
need help in blocking background in these cells. I have used normal serum,
peroxoblock, BSA, reagent A from Zymed's PAP kit (which is the recommended
kit for HCV. All of these in a variety of combinations. The 4%
paraformaldehyde fixed cells are false positive throughout. The MEOH fixed
cells are mostly stained around the periphery. IS ANYONE DOING HCV STAINING

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