Judy,

If you have a fluorescent microscope, fluorescent lysine-fixable 
dextrans can be used for this application.  The lysine on the dextran 
allows the fluorescent dextran to be fixed with aldehyde fixatives. 
You might check the Molecular Probes' web site for more info: 
www.probes.com

Karen in Oregon





>Date: Wed, 02 Aug 2000 14:54:05 -0700
>From: Judy Trogadis <judy@uhnres.utoronto.ca>
>Subject: live tissue stain
>To: histonet@pathology.swmed.edu
>
>We are trying to inject a compound into a live mouse.
>After 2 days the animal will be sacrificed and histology
>performed.  To ensure that the injections reached the
>appropriate site, we want to include a dye with the fluid
>for identification.
>
>Does anyone know of a stain which would be easily
>visible yet not interfere with the normal metabolism
>of the cells.
>
>Thank you.
>
>--
>Judy Trogadis
>Vision Science Research Program
>Toronto Western Research Institute
>399 Bathurst St.
>Toronto, ON M5T 2S8
>ph: 416-603-5088
>fax: 416-603-5126
>email:judy@uhnres.utoronto.ca