DAPI

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From:Jenny Molde <ctsmolde@samiot.uct.ac.za>
To:histonet@pathology.swmed.edu
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I have been asked to perform a nuclear stain for  macrophages on paraffin
embedded sections. Does anyone know of a fluorensence method I could use or
a protocol for DAPI? I am currently using an ABC technique with the Lectin
GSL-1 with good results. However, their are positive granules surrounding
the macrophages which we cannot figure out. Any contributions or advice
will be appreciated.





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