more GFP
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From: | Tamara Howard <howard@cshl.org> |
To: | Histology listserver <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Thu, 29 Apr 1999 09:43:04 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
One thing to add to the GFP discussion - you CAN do multiple GFP
expressions, for example, with YFP (yellow) and CFP (cyan), but you have
to be certain that your filters are tight enough. I don't have any
experience with paraffin processing of GFP, but I am finding that
immunodetection at the TEM level seems to be construct-dependent. Which
may just be me :), but it is VERY frustrating! Anyway, that may have some
bearing on the paraffin-processing variability; some constructs or mutants
of wild-type GFP may withstand fixation/dehydration better than others.
My pathetic $0.02!
Tamara Howard
CSHL
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