Re: mycobacterium detection in tissues (reply)

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Date:Fri, 23 Apr 1999 15:16:50 EDT
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have you tried the Fite's method for AFB?  It uses Xylene:Peanut Oil 50:50 
(two changes at 10 minutes each)  to deparaffinize, blot well, then Ziehl 
Neelson Carbol Fuschin for 30 minutes.  Decolorize in Acid Alcohol, rinse 
well, counterstain in Methylene Blue, let dry completely , dip in xylene and 

this may be just what you are looking for....check the AFIP manual.

debra flynn 
jackson, ms

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