Hi Karen,

We have used a rabbit anti M bovis (Dako) to detect mycobacterial antigens in fish granulomas.  
The mycobacteria are notoriously crossreactive so we don't have any way of speciating them.  
I'm not sure how well this will work as a screening method since not many fish will survive the 
fixation, embedding and sectioning. 

swimmingly yours

BJC - Brian

On Thu, 22 Apr 1999 14:52:02 -0800 Karen D. Larison wrote:

> From: Karen D. Larison <LARISONK@UONEURO.uoregon.edu>
> Date: Thu, 22 Apr 1999 14:52:02 -0800
> Subject: mycobacterium detection in tissues
> To: HistoNet@Pathology.swmed.edu
> 
> Hey gang,
> 
> Is there a way of detecting mycobacterium-containing granulomas in tissue 
> sections?  Mycobacterial infections are common in fish, and we would like to be 
> able to screen fish for it.
> 
> Thanks so much.
> 
> Karen Larison - University of Oregon
> 
>