Re: Sectioning bone (REPLY)
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From: | DFlynn9872@aol.com |
To: | andreah@imclone.com, Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 20 Apr 1999 22:26:49 EDT |
Content-Type: | text/plain; charset="us-ascii" |
Dear Andrea:
If you are looking at femoral head sections, remember that the decalcifying
process is a slow and tedious one. However, with proper monitoring, the end
result can be fabulous. I would recommend FIXING the specimen first in
formalin (depending on the size of the specimen) at least overnight. We use
Surgipath Decal I, and change it every day. It usually takes at least 4-5
days to decalcify a femoral head. Change the solution at least once daily,
and rinse the specimen daily for at least 30 minutes in tap water (to remove
calcium salts). When you finally can cut the specimen, and it is time to
process, make sure the tissue sections are no more than 2-3 mm thick.
After processing, embed the specimen diagonally in the mold. This keeps you
from "facing" into too much of the specimen at one time. I cut my blocks on
the vertical axis of the block holder on the microtome. After gently facing
the block, rub a little warm water over the surface and slowly section the
block. If it is still too hard, try immersing the block in cold decal
solution for 10-15 minutes after facing.
We fix bone marrow biopsies in formalin for 2 hours, then in DECAL I solution
for 30-45 minutes. RINSE WELL after decalcification. Results have been
great. (Again, I embed the specimen diagonally in the mold.)
I know this is way too long, but bottom line I think the key to good bone
sectioning is fixation and changing decal solutions periodically.
Good luck with the bone!
debra flynn
jackson, ms (sorry this was so long!) :)
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