Re: Rapid Immunoperoxidase

<< Previous Message | Next Message >> (Richard Maliniak), (HistoNet)
Date:Thu, 22 Apr 1999 10:02:20 -0400 (Eastern Daylight Time)


It is indeed possible to do "Rapid IHC".  

I have set up "most" of our IHC to work (quite well I might add ;-)) in
about an hour using the Dako Envision kit.  These are PCNA, BrdU, CD3,5,100
several other anti macrophage markers and several more.  Some that take more
time are p53 and LacC. 

I'm lucky enough that I have the time to work out optimal dilution and time
factors.  I also have control over fixation (usually) and work on mostly rat
tissues though I also do some mouse and human.  I don't have ANY control
over the human tissues so I sometimes have to do HIER on them.

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**

-- Begin original message --

> From: Richard Maliniak <>
> Date: Wed, 21 Apr 1999 13:39:22 -0700
> Subject: Rapid Immunoperoxidase
> To: HistoNet <>
> One of our Pathologists was at a meeting and one of lecturers
> {Italian} said ".....get the results of keratin immunostains on fresh
> frozen tissue 40 minutes after it is cut."   
>   I was asked to look into this to see if anyone does these rapid
> immunostains and if so is it a "homebrew" or is it a commerical kit. I
> know that there are immunostains that can be done in a few hours, but 40
> minutes from cutting to microscope is a bite short???
> Rick Maliniak

-- End original message --

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