Re: Limonene and pregnancy in lab, degrees

<< Previous Message | Next Message >>
From:"Jeff Silverman" <>
To:"Mark & Carrie Byrne" <>, "Yaskovich, Ruth A (NIDCR)" <RYaskovich@DIR.NIDCR.NIH.GOV>, "histonet" <>
Date:Fri, 30 Apr 1999 18:22:05 -0400
Content-Type:text/plain; charset=ISO-8859-1

Lets not forget that D-limonene is reported to have strong anti-cancer
activity. I'm glad I'm not allergic and find it a pleasure to use (no
commercial interests here at all!)  From  J Nutr 1999 Mar;129(3):775S-778S 
- d-limonene, which comprises >90% of orange peel oil, has chemopreventive
activity against rodent mammary, skin, liver, lung and forestomach cancers.
d-limonene also has chemotherapeutic activity against rodent mammary and
pancreatic tumors. As a result, their cancer chemotherapeutic activities
are under evaluation in Phase I clinical trials. 

Also see: Nutr Cancer 1998;32(1):1-7  Monoterpenes inhibit cell growth,
cell cycle progression, and cyclin D1 gene expression in human breast
cancer cell lines. 
Bardon S, Picard K, Martel P. 

Another good one: Cancer Chemother Pharmacol 1998;42(2):111-7  Phase I and
pharmacokinetic study of D-limonene in patients with advanced cancer.
Cancer Research Campaign Phase I/II Clinical Trials Committee. Vigushin DM,
Poon GK, Boddy A, English J, Halbert GW, Pagonis C, Jarman M, Coombes RC
 D-Limonene is a natural monoterpene with pronounced chemotherapeutic
activity and minimal toxicity in preclinical studies. A phase I clinical
trial to assess toxicity, the maximum tolerated dose (MTD) and
pharmacokinetics in patients with
advanced cancer was followed by a limited phase II evaluation in breast
cancer. METHODS: A group of 32 patients with refractory solid tumors
completed 99 courses of D-limonene 0.5 to 12 g/m2 per day administered
orally in 21-day cycles.
They concluded that D-Limonene is well tolerated in cancer patients at
doses which may have clinical activity. The favorable toxicity profile
supports further clinical

Here, people are INGESTING limonene. Of course, you need to check your MSDS
to be sure that there are no additives in the product you use. Also, since
limonene affects cell proliferation, this would be unwanted during a
pregnancy, where cell proliferation is what its all about. But it appears
likely that usage with normal precautions during pregnancy is harmless. 

Re: degree or no degree- I don't think anyone wants to take away any
non-degreed person's  HT or HTL but for the future, AS is probably going to
be the minimum for HT, although entry level salaries don't make this that
attractive- even less so for BS/HTL. In any career- related situation, it
is the individual's thirst for knowlege and understanding that determines
their ability to succeed and advance. True, attaining a degree (usually)
forces one to acquire these habits and abilities, but not always, and they
don't necessarily  persist past graduation. I got my HTL grandfathered as a
non-degreed HT with eight year's experience in 1983 and I have continued to
study, inquire and investigate and keep up with the literature on surgical
pathology and  IHC, also FISH, PCR and such, even though I've never done
these. It all in one's love for their chosen profession. I've currently got
a waitress from the local  diner helping me in histo- accesioning,
coverslipping, manual H&E and PAP stains and maintenance of the stains
(under my close supervision) as well as basic specials like alcian blue and
Diff Quick. She's picked it up quickly and does an excellent job. I've had
her cutting autopsies and fallopian tubes and she's pretty good at that
too. Without her help, I'd have to do it alone since the boss owns the lab
now and won't pay for a trained second person. 

Just my 2.5 cents, everyone have a lovely weekend.

> From: Mark & Carrie Byrne <>
> To: Yaskovich, Ruth A (NIDCR) <RYaskovich@DIR.NIDCR.NIH.GOV>; histonet
> Subject: RE: pregnancy in lab
> Date: Thursday, April 29, 1999 7:19 PM
> gee, a lab without chemicals for preggy techs....that would be nice but
> most of us only a pipe dream.
> we've had 2 techs pregnant in the last 2 years (i was one of
> no ill effects on the babies.....we did limit our exposure to as many
> chemicals as possible but otherwise no "extra" precautions were taken
> than our standard protective stuff.
> i believe that the limonene based xylene subs should be safe enough to
> (with gloves and a good fume hood, of course).  it is used in a number of
> cleaning products available in grocery/hardware/drug stores.
> carrie kyle-byrne
> salem hosp.

<< Previous Message | Next Message >>