Re: Immunos and Oven Temps/Times

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From:"Jeff Silverman" <peptolab@hamptons.com>
To:"Katri Tuomala" <katri@istar.ca>, "Elizabeth Morehead" <DREWSB@smtpgw2.musc.edu>
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Date:Sun, 18 Apr 1999 16:51:33 -0400
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I often leave paraffin sections to dry in a 60 degree C oven over the
weekend before immunostaining for ER/PR and stromal markers, no problems.
Also, admittedly my remarks about bunsen burners in histo need
clarification, its safer if you are only a one or two person lab- less
chance of forgetting or getting signals crossed. Our embedding usually
takes only 1/2 to 1 hour and then its off for the day.  
Jeff Silverman

----------
> From: Katri Tuomala <katri@istar.ca>
> To: Elizabeth Morehead <DREWSB@smtpgw2.musc.edu>
> Cc: Histonet@Pathology.swmed.edu
> Subject: Re: Immunos and Oven Temps/Times
> Date: Monday, April 19, 1999 10:49 AM
> 
> Elizabeth Morehead wrote:
> > 
> > Oh Blessed Gurus of Immunos...A moment of your time, please.
> > 
> > How long are you incubating your immuno slides?  What temp?
> > Anybody ever heard of leaving immuno control slides in a 70 degree oven
for the weekend?  Yes, I said WEEKEND!   Anybody doing anything special to
keep fat on slides (dermis and breast)?
> >  Your answers could very possibly win (or lose) me a bet and your
opinions are most appreciated!
> > 
> > Thanks in advance.
> > 
> > Beth
> > 
> > Beth Morehead
> > Med U of SC
> > (843) 792-4223
> 
> Hi Beth,
> I've never heard anyone leaving slides that long at 70C. Maybe 1-2
> hours. I do leave my slides in at 45C over the weekend with no ill
> effects as far as I can see.
> Katri
> 
> Katri Tuomala
> Anatomic Pathology
> St.Joseph's Hospital
> Hamilton, Ontario, Canada



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