Re: D profile knives

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From:"R.Wadley" <> (by way of histonet)
To:histonet <>
Date:Wed, 21 Apr 1999 02:31:15 -0400
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	Dear Mary Lou,

	I've always cut my JB4 section with disposable knives.  Initially
with ust
the normal knife as supplied by Leica & others, but finally with polymer
coated blades (cleaned in ethanol) by Sturkey.  These give nice sections in
the 2 -15 um range, depending on your microtome & your needs.  The other
alternative, depending on the size of your block is to use glass knives.  I
have successfully cut JB4 at 0.5 um on a glass knife, but 1 um is much
easier.  I assume Ralph knives would also be a good option.


	Rob W.

At 10:23 AM 4/19/99 -0400, you wrote:
>Hi Histoland,
>Anybody out there proficient in sharpening D knives? Tungsten carbide. I
>can't seem to polish the edge to give me a clear JB4 section. Shandon has
>been very helpful but I hoping there's another helpful hint I can't figure
>out. I'm afraid to up the degree any more than I have at the end.
>Thanks for any tips.
>Mary Lou at Cornell.
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
	(Under development)

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