Re: D profile knives
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Date: | Wed, 21 Apr 1999 02:31:15 -0400 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Mary Lou,
I've always cut my JB4 section with disposable knives. Initially
with ust
the normal knife as supplied by Leica & others, but finally with polymer
coated blades (cleaned in ethanol) by Sturkey. These give nice sections in
the 2 -15 um range, depending on your microtome & your needs. The other
alternative, depending on the size of your block is to use glass knives. I
have successfully cut JB4 at 0.5 um on a glass knife, but 1 um is much
easier. I assume Ralph knives would also be a good option.
Regards
Rob W.
At 10:23 AM 4/19/99 -0400, you wrote:
>Hi Histoland,
>
>Anybody out there proficient in sharpening D knives? Tungsten carbide. I
>can't seem to polish the edge to give me a clear JB4 section. Shandon has
>been very helpful but I hoping there's another helpful hint I can't figure
>out. I'm afraid to up the degree any more than I have at the end.
>
>Thanks for any tips.
>
>Mary Lou at Cornell.
>
>
>
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.unsw.edu.au/clients/microbiology/CAF.html
(Under development)
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