Hi,
I have an over eager visiting fireman...he did Brdu on slides meant for
apopotosis.  I am sure this is the dumbest question yet...but is it
possible to re-stain or combine the two...ok...go ahead and laugh.  Wish I
knew how to make this anonymous.
Trisha
On Tue, 27 Apr 1999, Technical Services wrote:

> Hi Cindy.
> 
> We have a double-stain kit at DAKO which utilizes our Envision polymer
> system, and includes a blocking reagent that enables you to completely carry
> out one stain, eliminate any vestige of it (aside from the colorimetric
> reaction of course) and then proceed with another stain. You can use two
> polyclonals, two monoclonals, what have you. I've spoken with people who
> have even quadruple stained. If you would like more information please
> contact me.
> 
> Thank you,
> 
> Joel Weisenberger
> DAKO Corporation
> Technical Services
> 800-235-5743 x5325
> techserv@dakousa.com
> 
> 
> -----Original Message-----
> From: Cindy Chard-Bergstrom <CHARD_B@vet.ksu.edu>
> To: Histonet@Pathology.swmed.edu <Histonet@Pathology.swmed.edu>
> Date: Tuesday, April 27, 1999 9:59 AM
> Subject: Double Staining
> 
> 
> >Dear Histonetters,
> >
> >    I will soon be working on a double staining IHC project. I seem
> >to remember someone discussing a proceedure that involved stripping
> >off the first primary, after addition of the chromogen, and then
> >applying the second primary. Does anyone have this protocol or any
> >others involving double staining. One project involves two rabbit
> >primary antibodies and there is not enough antibody to conjugate
> >either one to HRPO or alk-phos. Thanks to all in advance.
> >
> >Cindy Chard-Bergstrom, BS, HT(ASCP)
> >
> >
> >
> >
> >
> 
> 
> 

Trisha Emry 
U of Washington, Seattle