Hi Cindy.

We have a double-stain kit at DAKO which utilizes our Envision polymer
system, and includes a blocking reagent that enables you to completely carry
out one stain, eliminate any vestige of it (aside from the colorimetric
reaction of course) and then proceed with another stain. You can use two
polyclonals, two monoclonals, what have you. I've spoken with people who
have even quadruple stained. If you would like more information please
contact me.

Thank you,

Joel Weisenberger
DAKO Corporation
Technical Services
800-235-5743 x5325
techserv@dakousa.com


-----Original Message-----
From: Cindy Chard-Bergstrom <CHARD_B@vet.ksu.edu>
To: Histonet@Pathology.swmed.edu <Histonet@Pathology.swmed.edu>
Date: Tuesday, April 27, 1999 9:59 AM
Subject: Double Staining


>Dear Histonetters,
>
>    I will soon be working on a double staining IHC project. I seem
>to remember someone discussing a proceedure that involved stripping
>off the first primary, after addition of the chromogen, and then
>applying the second primary. Does anyone have this protocol or any
>others involving double staining. One project involves two rabbit
>primary antibodies and there is not enough antibody to conjugate
>either one to HRPO or alk-phos. Thanks to all in advance.
>
>Cindy Chard-Bergstrom, BS, HT(ASCP)
>
>
>
>
>