Re: Citrate Buffer recipe

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From:Katri Tuomala <>
To:Chappell Joyce <>
Date:Thu, 29 Apr 1999 22:34:46 -0400
Content-Type:text/plain; charset=us-ascii

Chappell Joyce wrote:
> Hi Histonetters,
> I need a recipe to make my own citrate buffer antigen retrieval solution.
> Thanks in advance.
> Marsha Price

Hi Marsha,

The citrate buffer recipe for antigen retrieval:

  citric acid           2.1 g
  distilled water       1000 ml
Adjust to pH 6.0 by adding 2M sodium hydroxide

I use the same solution for about 4-5 times and then make it up fresh

Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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