Hi Karen,

I cannot offer information on fish specifically, but you should beware of 
immunohistochemistry using antibodies against mycobacteria, because they 
have been shown to stain other agents as well (incl. gram positive bacteria 
and fungi)(Geisel et al, J. Vet. Med. B. 41, 548-553 1994).

Cindy Farman

-----Original Message-----
From:	Karen D. Larison [SMTP:LARISONK@UONEURO.uoregon.edu]
Sent:	Thursday, April 22, 1999 3:52 PM
To:	HistoNet@Pathology.swmed.edu
Subject:	mycobacterium detection in tissues

Hey gang,

Is there a way of detecting mycobacterium-containing granulomas in tissue
sections?  Mycobacterial infections are common in fish, and we would like 
to be
able to screen fish for it.

Thanks so much.

Karen Larison - University of Oregon