There are some immunohistochemical methods that don't require any
profussion of the animal.  We use an antibody to collagen IV which stains
the endothelial cells lining vessels very nicely.
Patsy Ruegg

-----Original Message-----
From: Fernando Capela e Silva
To: histonet@Pathology.swmed.edu
Sent: 4/25/99 8:10 AM
Subject: VASCULARIZATION

I would like if someone information about techniques to study tissue
vascularization in paraffin blocks: what kind of injection in the
experimental animal and the best method of stain.

With compliments
Fernando Capela e Silva
Departamento de Biologia
Universidade de Evora
Apartado 94
7002-554 Évora
PORTUGAL

Telephone: +351-66-740 98 81
Telefax: +351-66-711 231
E-mail: fcs@uevora.pt
http://home.dbio.uevora.pt/~fcs/FCAPELAeSILVA.html