RE:Mouse on Mouse
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From: | Matthew Ogdie <mogdie@innogenex.com> |
To: | "'HistoNet@Pathology.swmed.edu'" <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 16 Apr 1999 10:04:59 -0700 |
Content-Type: | text/plain; charset="us-ascii" |
Hello,
InnoGenex has a 'Mouse-on-Mouse' system which works consistently on all
mouse tissue, including Ig saturated tissue (spleen,vascular tissue, etc)
and tissue w/ endogenous biotin(kidney). The "ISO-IHC" kit utilizes a
labelling reagent that exclusively labels mouse primaries----I've used the
kit on spleen(Anti-Ribonuclear protein), and it produced absolutely no
background....(better signal:noise ratio than I usually get performing
standard IHC on human tissue)........There are other options--Ig blocks
sold by many vendors (consensus seems to side with Pharmingen), making your
own block, etc......but we really haven't found results that are
competitive with the ISO-Kit.
Good Luck,
Matthew Ogdie
IGX
(925) 543-1414
----------
From: Blanca Arango[SMTP:blanca_arango@yahoo.com]
Sent: Friday, April 16, 1999 7:51 AM
To: 'Histonet'
Subject: Mouse in mouse
Hi Histonetters,
I am looking for the expression of p27-protein in mouse retina using an
anti-p27 MOUSE-antibody. To develop the reaction I use the DAB system,
but I find false positives in the control slides (without first
antibody). I work in formalin-fixed, plastic embedded tissue sections.
My protocol includes trypsin digestion
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