RE:Mouse on Mouse

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From:Matthew Ogdie <>
To:"''" <>
Date:Fri, 16 Apr 1999 10:04:59 -0700
Content-Type:text/plain; charset="us-ascii"


InnoGenex has a 'Mouse-on-Mouse' system which works consistently on all 
mouse tissue, including Ig saturated tissue (spleen,vascular tissue, etc) 
and tissue w/ endogenous biotin(kidney). The "ISO-IHC" kit utilizes a 
labelling reagent that exclusively labels mouse primaries----I've used the 
kit on spleen(Anti-Ribonuclear protein), and it produced  absolutely no 
background....(better signal:noise ratio than I usually get performing 
standard IHC on human tissue)........There are other options--Ig blocks 
sold by many vendors (consensus seems to side with Pharmingen), making your 
own block, etc......but we really haven't found results that are 
competitive with the ISO-Kit.

Good Luck,
Matthew Ogdie
(925) 543-1414

From: 	Blanca Arango[]
Sent: 	Friday, April 16, 1999 7:51 AM
To: 	'Histonet'
Subject: 	Mouse in mouse

Hi Histonetters,
I am looking for the expression of p27-protein in mouse retina using an
anti-p27 MOUSE-antibody. To develop the reaction I use the DAB system,
but I find false positives in the control slides (without first
antibody). I work in formalin-fixed, plastic embedded tissue sections.
My protocol includes trypsin digestion 

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