RE: Melanin

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"'HistoNet@Pathology.swmed.edu'" <HistoNet@Pathology.swmed.edu>, "'Jean-Hugues Deschesnes'" <dspjhd@cha.quebec.qc.ca>
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Date:Mon, 26 Apr 1999 08:37:18 -0400
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Jean-Hugues,

Whenever I have a problem with melanin bleach techniques, I resort to
the slower, but tried and true, Mayer technique. This was the first
melanin bleach I learned more than 40 years ago!
Culling, Ed.4. p284 gives the following;
1) place a thin layer(3-5mm) of potassium chlorate at the bottom of a
coplin jar and fill with 70% ethanol. 
2) place hydrated section in the jar.
3) slowly add 1ml Conc. HCl to the bottom of the coplin jar.
The nascent chlorine produced will bleach melanin within a few hours.(I
leave the slides overnight)

Before all the safety guru's jump all over me, let me add, that this
should be performed in a fume hood and yes, pot. chlorate is potentially
explosive!!  However, the method works very well!

Bryan

>----------
>From: 	Jean-Hugues Deschesnes[SMTP:dspjhd@cha.quebec.qc.ca]
>Sent: 	April 23, 1999 1:46 PM
>To: 	HistoNet@Pathology.swmed.edu
>Subject: 	Melanin
>
>We have been trying to bleach melanin using KMNO4 or hydrogen peroxyde
>(30%) but this is hard on tissues. Has anyone had success with other
>technics.
>Thank you
>
>Jean-Hugues
>
>
>



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