RE: Immunos and Oven Temps/Times

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From:Alex Brown <AlexB@nayrshire.scot.nhs.uk>
To:"'Histonet'" <Histonet@Pathology.swmed.edu>
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Date:Mon, 19 Apr 1999 11:59:00 +0100
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Hi Beth,
	We normally dry our immuno slides overnight at 37deg and then 1
hour at 60deg.  As for keeping any kind of sections on slides, crossing
your fingers ( and legs, and toes ) may help. I don't think there is a
foolproof method, and to an extent it makes a big difference if your
blocks are properly processed and infiltrated. We tend to use
Poly-L-Lysine coated slides in general, and 'charged' slides for the
more troublesome ones. Which come to think of it seems to be most of
them nowadays :)
	I blame the person(s) who came up with the idea of microwaving.
Good results, but a pain in the **s.  As for incubation times, all ours
are at room temperature. The times vary with the antibody ( and dilution
). Most are based around a 2 hour primary incubation for paraffin
sections.
	Hope this helps
		Alex Brown
		Kilmarnock, Scotland.
P.S.  Never been a 'Guru' before,  :)

 ----------
From: Elizabeth Morehead
To: Histonet@Pathology.swmed.edu
Subject: Immunos and Oven Temps/Times
Date: 17 April 1999 17:48

Oh Blessed Gurus of Immunos...A moment of your time, please.

How long are you incubating your immuno slides?  What temp?
Anybody ever heard of leaving immuno control slides in a 70 degree oven
for the weekend?  Yes, I said WEEKEND!   Anybody doing anything special
to keep fat on slides (dermis and breast)?
 Your answers could very possibly win (or lose) me a bet and your
opinions are most appreciated!

Thanks in advance.

Beth

Beth Morehead
Med U of SC
(843) 792-4223




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