Marsha,
	If you don't already have it - 10mM citrate Buffer can be made
differently here is what I do:

	Stock Solns:
		0.1M Citric Acid
			1.92 gm citric acid, anhydrous to 100ml in glass
distilled water
		0.1M NaCitrate, dihydrate
			14.7 gm NaCitrate dihydrate to 500ml in glass
			distilled water

	Working sloution:
		9.0 ml of 0.1 M Citric Acid
		41.0 ml of 0.1M NaCitrate, dihydrate
		bring final volume to 500 ml with glass distilled water
		pH to 6.0 with 5N NaOH
 
As you can see you can make as much or as little as you like. I keep the
stocks at 4C and make the working volume dependant on what is going on hta
week. Be sure to bring to RT before using.


Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov

		

> ----------
> From: 	Chappell Joyce[SMTP:cjpa@bridgewater.net]
> Sent: 	Wednesday, April 28, 1999 11:04 AM
> To: 	HistoNet
> Subject: 	Citrate Buffer recipe
> 
> Hi Histonetters,
> I need a recipe to make my own citrate buffer antigen retrieval solution.
> Thanks in advance.
> Marsha Price
> 
> 
>