NALT isolation/removal

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From:Gayle Callis <>
Date:Mon, 26 Apr 1999 10:55:58 -0600

There are two excellent publications on isolation and removal of murine
NALT, nasal associated lymphoid tissue.

1.  Please try to look at this article IN THE JOURNAL, as the photo
reproduces poorly.  

Asanuma, H et al  Isolation adn characterization of mouse nasal associated
lymphoid tissue  J Immunol Methods  202:123-131, 1997

2.  This article has an excellent diagram, without photos, on exact location
of NALT in relation to the first molars.  

Heritage PL et al, Comparison of murine nasal associated lymphoid tissue and
peyers patches Am J Respir Crit Care Med 156:1256-1262, 1997

Dissecting NALT out of mouse is not difficult, 
just takes some practice for it to come out intact
Use care and good instruments.  It tends to curl, since it is attached just
above and to the soft palate.  If you need help on how to snap freeze this
tissue, email me.  We do a different dry ice freezing to keep it totally 

Good luck, and hope this helps.

Gayle Callis  


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