Hi Histonetters,
I am looking for the expression of p27-protein in mouse retina using an
anti-p27 MOUSE-antibody. To develop the reaction I use the DAB system,
but I find false positives in the control slides (without first
antibody). I work in formalin-fixed, plastic embedded tissue sections.
My protocol includes trypsin digestion and microwave antigen retrieval.
I have already
tried endogenous peroxide activity blocking and endogenous biotin
blocking. 
Does anyone have a solution?
Does anyone have any experience in blocking of endogenous Ig?

Thanks for your input.

Blanca Arango Gonz#225#lez 
University Eye Hospital Tuebingen
Experimentelle Ophthalmology
Roentgenweg 11 
D-72076 Tuebingen 
Germany   
Tel.: +49 - 07071 - 29 87425 
Fax: +49 - 07071 - 29 5777

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