GFP/fixation/paraffin sections

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From:Gayle Callis <>
Date:Wed, 28 Apr 1999 11:24:19 -0600

I had (much to my happiness!) two replies from people who had used
GFP with SUCCESS in formalin fixed tissues/paraffin processing.  

Could it be that the GFP they are using is a stable form (heat stabile,
resistant to solvents?).  If so I am hoping they share the specifics
of what was done, exact GFP used (there are chimeras out there!) and
some of the processing times/solvents/paraffin.  

At least we had one thing in common, formalin fixation of tissues, time
of fixation may be a big factor also.

ladies, are you willing to share these goodies, it would be a big help.

Gayle Callis

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