GFP/fixation/paraffin sections
<< Previous Message | Next Message >>
From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 28 Apr 1999 11:24:19 -0600 |
Content-Type: | |
I had (much to my happiness!) two replies from people who had used
GFP with SUCCESS in formalin fixed tissues/paraffin processing.
Could it be that the GFP they are using is a stable form (heat stabile,
resistant to solvents?). If so I am hoping they share the specifics
of what was done, exact GFP used (there are chimeras out there!) and
some of the processing times/solvents/paraffin.
At least we had one thing in common, formalin fixation of tissues, time
of fixation may be a big factor also.
ladies, are you willing to share these goodies, it would be a big help.
Gayle Callis
<< Previous Message | Next Message >>