Fontana Masson Silver stain

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Date:Fri, 23 Apr 1999 16:30:10 -0500
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On Monday I have to do the Fontana-Masson stain and I haven't done it in a long
time.  I checked the AFIP manual and the Sheehan book, and the timing is very
different in each book.  For instance the AFIP says to stain in the silver soln.
for 1 hour, and Sheehan says two hours.
Also thegold chloride ranges from 2 to 3  minutes in Sheehan to 10 minutes in
the AFIP. Hypo times range from 1 minute to 5 minutes.  Does anyone do this
stain routinely enough to give me some guidelines?     I am doing this on
pancrea tumors if that makes any difference. Thanks for any help you can give
Karen Dulany HTL
Eppley Institute for Cancer Research
Omaha, NE

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