2) Mice IHC

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From:"Masayuki Miyagishima, MD" <mmiyagis+@pitt.edu>
To:HistoNet@Pathology.swmed.edu
Reply-To:
Date:Sat, 17 Apr 1999 14:41:17 -0500
Content-Type:text/plain; charset=iso-2022-jp

Hi, all histoneters, thank you very much for providing me a lot of good
suggestions.  Let me summarize the information I received.

2) Mice IHC
Some people suggested me nonserum-blocking works better than using serum
blocking.
I decided to order Innovex mouse IHC kit.  I chose Innovex Biosciences
only because the person I talked with on the phone at the technical
support in Innovex had the best knowledge on mice IHC, and seemed to be
the most helpful.  The one I chose is HistSTAT (rabbit, mouse) NB312-L. 
I chose it because I use frozen sample and expect to suffer from biotin
background.  HistSTAT is not biotin-system, so I chose it.  I will let
you know how the things come out.

-- 
Masayuki Miyagishima, MD
University of Pittsburgh, 
Department of Surgery
412-647-2345, and ask the hospital operator to page 3228, please.



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