whole mammary mounts-mouse

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From:"Kowsz, Kim P" <kim_p_kowsz@groton.pfizer.com>
To:"'HistoNet@Pathology.swmed.edu'" <HistoNet@Pathology.swmed.edu>
Date:Tue, 13 Apr 1999 13:07:16 -0400
Content-Type:text/plain; charset="iso-8859-1"

I am trying to do whole mammary mounts on mice.  The tissue is fixed on the
slide in Carnoy's for 2-4 hours before being stained in carmine alum.  My
sections come out very thick even though the tissue is "squashed" between
two glass slides before fixation.  Is there a de-fatting step that some
people use to thin the section?  Is there a more specific technique to
spread the mammary tissue on the glass slide?  Thanks for any help.


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