I am trying to do whole mammary mounts on mice.  The tissue is fixed on the
slide in Carnoy's for 2-4 hours before being stained in carmine alum.  My
sections come out very thick even though the tissue is "squashed" between
two glass slides before fixation.  Is there a de-fatting step that some
people use to thin the section?  Is there a more specific technique to
spread the mammary tissue on the glass slide?  Thanks for any help.

Kim_P_Kowsz@groton.pfizer.com