re:cd-5
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| From: | Jim Manavis <jim.manavis@imvs.sa.gov.au> |
| To: | Histonet <HistoNet@Pathology.swmed.edu> |
| Reply-To: | |
| Date: | Fri, 09 Apr 1999 08:33:36 +0930 |
| Content-Type: | text/plain; charset="iso-8859-1" |
On our FFPE material we use a CD-5 from Becton Dickinson (clone leu-1) at a
dilution of 1:10 with TRS microwave retrieval, and obtain very good results.
As far as Cyclin D1 is concerned we use the Novocastra (NCL-CYCLIN D1-GM)
antibody at a dilution of 1:60 with TRS microwave retrieval, Trypsin, and
enhancement with Imidazole. Works beautifully.
Jim Manavis
Neuropathology & ICC Lab
Dpt Tissue Pathology
IMVS
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