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From:Jim Manavis <>
To:Histonet <>
Date:Fri, 09 Apr 1999 08:33:36 +0930
Content-Type:text/plain; charset="iso-8859-1"

On our FFPE material we use a CD-5 from Becton Dickinson (clone leu-1) at a
dilution of 1:10 with TRS microwave retrieval, and obtain very good results.

As far as Cyclin D1 is concerned we use the Novocastra (NCL-CYCLIN D1-GM)
antibody at a dilution of 1:60 with TRS microwave retrieval, Trypsin, and
enhancement with Imidazole. Works beautifully.

Jim Manavis
Neuropathology & ICC Lab
Dpt Tissue Pathology

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