Undecalcified tissue for IHC...HELP!

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From:Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
Date:Thu, 08 Apr 1999 12:54:27 +1000
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Hi my name is Doug & I'm a student in Zoology doing my PhD & am having to do some IHC on fresh frozzen tissue.I will be embedding the tissue in OCT for cryosectioning BUT there are some areas of undecalcified bone/cartilage.My question is ,can anyone please suggest a a decalcification method that has the least harmful effect on antigens but is faster acting than EDTA?? Note:tissue is prefixed in 4% PFA & now in 20% sucrose @ 4C temp.Thankyou in advance for your help...& I look forward to your very helpful replies.Cheers...Doug in Oz!!

DEPARTMENT of ZOOLOGY	         *  ph:    +61 3 93446282
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