Undecalcified tissue for IHC...HELP!
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From: | Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au> |
To: | histoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 08 Apr 1999 12:54:27 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Hi my name is Doug & I'm a student in Zoology doing my PhD & am having to do some IHC on fresh frozzen tissue.I will be embedding the tissue in OCT for cryosectioning BUT there are some areas of undecalcified bone/cartilage.My question is ,can anyone please suggest a a decalcification method that has the least harmful effect on antigens but is faster acting than EDTA?? Note:tissue is prefixed in 4% PFA & now in 20% sucrose @ 4C temp.Thankyou in advance for your help...& I look forward to your very helpful replies.Cheers...Doug in Oz!!
--
BRUCE ABALOZ
HISTOLOGIST
DEPARTMENT of ZOOLOGY * ph: +61 3 93446282
The UNIVERSITY of MELBOURNE * fax: +61 3 93447909
Parkville Victoria.3052 * email: b.abaloz@zoology.unimelb.edu.au
AUSTRALIA.
IF YOU WANT TO BE HAPPY......BE!!
I DO NOT SEEK............I FIND!!
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