Re: warthin starry stain

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From:Kathy Wortham <>
To:Gerard Spoelstra <>, HistoNet Server <>
Date:Mon, 05 Apr 1999 08:21:11 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

I'm not sure if you wanted to know if you can reuse the solutions or
how long they last, so I will try to answer both.
You cannot reuse the solutions once they have been heated and the
developer should be mixed together immediatly before use, then
The solutions should be kept in the refrigerator, even the gelatin(just
heat in the microwave to melt before use). The solutions should be fine
for 3-4 months.

I hope this answers your questions.

Kathy Wortham
USDA, Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605

--- Gerard Spoelstra <> wrote:
> Kathy
> I use a microwave version of the bosma-steiner
> method for spirochaetes but
> I'd like to give your version of warthin starry
> method a try. How long can
> you the solutions for the method? Thanks.
> Gerard Spoelstra
> Histopathology
> Division of Veterinary and Biomedical Science
> Murdoch University
> Western Australia


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