Hi,
I have inherited 100 or more boxes of very large slides that I now want to
use for Brdu and Apoptosis work.  When I look at the slides I can see the
spots from old oil coatings that have broken down (so I was told) and I
want to clean them and coat them with silane.

How can I clean these slides and prepare them for the coating procedure?
I don't want to do them one at a time...I might as well buy the new ones
rather than spend that much time on them.

Thanks,
Trisha
U of Washington, Seattle



On Mon, 5 Apr 1999, Kathy Wortham wrote:

> I have tried just about all the coated or charged slides on the market.
> My choice is still the silane coated slides I do myself in the lab. I
> know it takes a good bit of time, but I have never had any problems
> with anything coming off. I can't say that about the commercially
> available ones!
> 
> Kathy Wortham
> USDA, Food Safty Inspection Service
> 950 College Station Road
> Athens, Ga 30605
> --- "Masayuki Miyagishima, MD" <mmiyagis+@pitt.edu> wrote:
> > > >We use BD gold Seal Ultrastick slides (Erie
> > Scientific or Fisher Scientific)
> > 
> > Another question.  I am dealing with a tiny mice
> > frozen section on
> > SuperFrostPlus slide.  The tissue still comes off
> > while IHC.  I was not
> > drying enogh before the IHC, because I thought
> > drying will reduce the
> > antigenicity of the frozen section (perfusion
> > fixed.).  Should I dry the
> > sections completely to get better attachment on the
> > slide?
> > 
> > Or is this BD gold Seal Ultrastick slides better
> > than Superfrost?
> > What is the ultimate super sticky slide under the
> > sun???
> > 
> > -- 
> > Masayuki Miyagishima, MD
> > University of Pittsburgh, 
> > Department of Surgery
> > 412-647-2345, and ask the hospital operator to page
> > 3228, please.
> > 
> > 
> 
> ===
> 
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