Re: mice frozen tissue IHC protocol.

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From:"Masayuki Miyagishima, MD" <>
To:"Masayuki Miyagishima, MD" <>
Date:Sat, 03 Apr 1999 22:51:53 -0500
Content-Type:text/plain; charset=iso-2022-jp

Thank you very much for everybody! 
Everybody is so kind!

Let me specificall ask several more basic questions.

To stain mice tissue, 
1) If you have choice,  which primary antibody do you chose, rat, rabbit
or goat, etc?
2) Again, which secondary antibody do you chose?  For example, if you
choose rabbit primary antibody, do you choose goat anti-rabbit or rat
3)If you go for fluorescense, which fluorescent conjugated secondary is
your choice, Alexa, Fit-C, Cy3, etc?
4) If you go for peroxidase, which secondary is your choice (product
5) For blocking solution, what is your strategy?

Am I asking too much??

Masayuki Miyagishima, MD
University of Pittsburgh, 
Department of Surgery
412-647-2345, and ask the hospital operator to page 3228, please.

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