Re: endogenous peroxidase,

<< Previous Message | Next Message >>
From:Tim Morken <timcdc@hotmail.com>
To:HistoNet@Pathology.swmed.edu
Reply-To:
Date:Tue, 06 Apr 1999 08:11:27 -0400 (EDT)
Content-Type:text/plain

Bruce,

You are right in thinking that you can probably eliminate the h2o2 in 
brain tissue IHC. It is mostly blood cells that cause the problem. In 
perfused tissue it may be even less of a problem due to lack of leaching 
that occurs between time of cell death and fixation. 

In fact, in any preparation in which you feel the h202 may cause 
problems, you can put up with endogenous peroxidase staining after 
determining what is endogenous and then ignoring it when evaluating the 
slides.

Some even use the endogenous staining to their advantage as proof the 
DAB (or other chromogen)is working correctly.

Tim Morken, B.A., EMT(MSA), HTL(ASCP) 
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043







----Original Message Follows----
From: Bruce A Rasmussen <brasmuss@osf1.gmu.edu>
To: HistoNet@Pathology.swmed.edu
Subject: endogenous peroxidase,
Date: Mon, 05 Apr 1999 18:02:34 -0400 (EDT)


Is background staining caused by endogenous peroxidase mainly a blood
confound? In other words with well perfused brain tissue is H202
unnecessary or not worth the tissue damage and loss of immunoreactivity?
Many thanks,
Bruce



--------------------------------------
Bruce Rasmussen
Predoc Fellow in Experimental Neuropsychology
The Krasnow Institute for Advanced Study
George Mason University
Mail Stop 2A1
Fairfax, VA 22030-4444
Office:703-993-4358
Lab:703-993-4369
Home:703-765-4570
Fax:703-993-4325
---------------------------------------






Get Your Private, Free Email at http://www.hotmail.com



<< Previous Message | Next Message >>