Re: cleaning knives, Prefer unclean
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From: | Jeff Silverman <peptolab@hamptons.com> |
To: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
Reply-To: | |
Date: | Wed, 07 Apr 1999 18:09:06 -0400 |
Content-Type: | text/plain; charset=ISO-8859-1 |
Clean blades?? Never heard of such a thing. My AccuEdge low profile are at
first hypersharp sometimes, causing rolling of the first few sections but
facing something soft or waiting for a few sections gets the ribbons
flowing. Also, gentle hot breath (like fogging a mirror) on the block as
you cut never fails to form a ribbon. Looks dumb but it works. Also, I
never knew of the invincibility of 1% periodic acid. I prefer to stain the
slides dropwise on the counter using fresh periodic and Schiff each time.
Of course, in my dinky little hospital I never have to stain more than five
at once anyway.
Jeff Silverman
----------
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> To: histonet@pathology.swmed.edu
> Subject: cleaning knives, more comments
> Date: Wednesday, April 07, 1999 3:40 PM
>
> I have found this discussion interesting. I wonder what the vendors have
> to say about this? Also, if they give the advice, is it because they
> have direct experience with "knife cleaning" and daily sectioning, or is
> this blanket concrete evidence advice?
>
> I find comments about a knife being too sharp, you have to dull it an
> interesting comment. I have never found a knife too sharp for my
> tastes/usage. I section routinely at 2 um, with an "unclean" blade,
> without any problems (Accuedge, high profile, and have used the low and
> a whole array of vendor manufactured blades. Also, if you clean knives
for
> paraffin sections and not frozens, why? If oil is the problem, would
there
> be a difference? And if it is a sharpness problem why would you need
> duller blades for paraffin vs the frozens?
>
> I always felt (what is often construed as too sharp a blade??) that the
> differences in knife angles, even between lots of knives, was more of
> a difference, rather than a knife that is too sharp. If I have problems
> cutting a block, with a brand new knife, it often is a slight tweak of
the
> holder to fine adjust that takes care of the problem. Other factors are
> paraffin, tissue, and how it was processed. I also found that a section
> not forming correctly on a new blade was also linked to a truly square
> edge on the block (un- bless those rounded corners on embedding molds!,
> love the peel away molds, square corners!) I often reshape rounded
> corners, and all goes well on a brand unclean blade.
>
> so if cleaning is of prime importance, it would be to remove oil, and
> the sharper the blade, the better I like it. After all, I don't dull my
> glass knives before use, and like my disposables to approximate these
> blades as much as possible. I did go through a time of cleaning
disposables,
> but overall found it didn't make any difference, and actually preferred
the
> "unclean" Could that have been damage to the coating of the edge,
teflon,
> and whatever else has been used? That is a good point.
>
> I have seen blades with too much oil gunk, wondered about those, but
> never have had a section fall off from oil contamination of slide,
> Dirty slides - yes or mungey fingerprints on slide surface - sections
didn't
> like oily skin. One study required NO fat carryover from slide to slide,
and
> that did require cleaning a knife between blocks, and everything else in
the
> vicinity of blade inside a cryostat.
>
> Sorry for the tirade, but have found this whole discussion educational,
> At the risk of being a very "dull" person, I remain in the "unclean"
camp!
>
> Gayle Callis
>
>
>
>
>
>
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