Re: PAS problems
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From: | Katri Tuomala <katri@istar.ca> |
To: | Donna Carr <dkc@odsgc.net> |
Reply-To: | |
Date: | Wed, 07 Apr 1999 22:13:04 -0400 |
Content-Type: | text/plain; charset=us-ascii |
Donna Carr wrote:
>
> Dear Fellow Histonetters,
> We are currently experiencing problems with our PAS/DPAS stain, We
> have replaced the Schiff reagent. And have tried different controls. I
> would greatly appreciate any advice on what tissues you use as controls
> and methods of staining. This is a recent problem that leads us to
> believe it is either a contaminated reagent or bad control. Any input
> is welcome. Thanks Donna
Hi Donna,
We use autopsy liver tissue as a control. Just verify the presence of
glycogen with a known control before use, not all liver tissues have
detectable amount of glycogen present.
Katri
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