Re: PAS problems

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From:Katri Tuomala <>
To:Donna Carr <>
Date:Wed, 07 Apr 1999 22:13:04 -0400
Content-Type:text/plain; charset=us-ascii

Donna Carr wrote:
> Dear Fellow Histonetters,
>     We are currently experiencing problems with our PAS/DPAS stain,  We
> have replaced the Schiff reagent. And have tried different controls.  I
> would greatly appreciate any advice on what tissues you use as controls
> and methods of staining.  This is a recent problem that leads us to
> believe it is either a contaminated reagent or bad control.  Any input
> is welcome.   Thanks Donna

Hi Donna,
We use autopsy liver tissue as a control. Just verify the presence of
glycogen with a known control before use, not all liver tissues have
detectable amount of glycogen present.


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