Re: IHC endogenous peroxidase block

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From:Aaron Levin <>
To:Cynthia Favara <>, "'HistoNet Server'" <>
Date:Fri, 02 Apr 1999 11:16:30 -0800
Content-Type:text/plain; charset="iso-8859-1"

Dear Cynthia,

Endogenous peroxidase activity should be blocked prior to the 1st antibody
incubation. I recommend that the endogenous peroxidase activity be blocked
following deparaffinization and prior to antigen retrieval. For paraffin
embedded tissues endogenous blocking reagent can be prepared by adding 1
part of 30% hydrogen peroxide to 9 parts absolute methanol (mix well). The
tissue should be incubated in solution for 10 minutes at room temperature.

Best regards,

Aaron Levin
Zymed Laboratories

-----Original Message-----
From: Cynthia Favara <>
To: 'HistoNet Server' <>
Date: Friday, April 02, 1999 8:13 AM
Subject: IHC endogenous peroxidase block

> Curious as to when people block for endogenous peroxidase. Before or
>after 1st/link antibody. Has anyone done any experiments for epitope
>destruction caused by the peroxidase block?
>Thanks for the input.
>Cynthia Favara
>Rocky Mountain Laboratories
>903 S 4th Street
>Hamilton, MT 59840
>ph: 406-363-9317
>FAX: 406-363-9286

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