Re: Filipin for cholesterol
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | "Yandl, Emily" <eyandl@genzyme.com>, histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 14 Apr 1999 23:21:35 -0400 (EDT) |
Content-Type: | text/plain; charset="us-ascii" |
At 05:04 PM 4/12/99 -0400, you wrote:
> I recently tried the fluorescent Filipin stain for cholesterol, as
>found in Bancroft and Stevens. Problem is, I'm having a hard time finding
>any information about how the stain works.
The filipin molecule attaches to cholesterol in cell membranes,
and has been used as a label in electron microscopy. For a paper
reporting its use, see:
Pelletier, M & Vitale ML 1994. Filipin vs enzymatic localization of
cholesterol in guinae pig, mink and mallard duck testicular cells.
J. Histochem. Cytochem. 42(12): 1539-1554.
This is a paper in which filipin is used. The references might send
you back to some of the basic science of the method. Sorry I
can't provide anything better than this. (Perhaps Bancroft & Stevens
has some refs - I don't own the latest edition so can't immediately
check.)
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