Re: Factor XIIIa reply

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From:Keith Miller <rmkdhkm@ucl.ac.uk>
To:Neil Hand <mpznhand@unix.ccc.nottingham.ac.uk>
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Date:Tue, 13 Apr 1999 13:34:40 +0000
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Dear Neil,

Try Biogenesis [UK]  Tel: 01202 660006. They have several Factor XIIIa
antibodies, but we are currently using  code 4400-7708 with some success
after pretreating in Dako pH9.9 target retrieval fluid. Our retrieval
method is as follows:

Heat from cold for 25 minutes in 600 ml of retrieval fluid at full power in
850watt microwave oven.  After heating time has elapsed, transfer hot
container to sink of cold running water....then after about 10 minutes
transfer slides to cold running water. The saved retrieval fluid can be
topped-up and used again.

I hope this helps.

Keith







At 12:16 13/04/99 +0400, you wrote:
>I am looking for a supplier (preferably one in the UK) of the antibody
>Factor XIIIa to work on human tissue, but have been unable to locate it in
>Linscott.  Any suggestions would be much appreciated.
>
>Thanks in advance,
>
>Neil Hand,
>Histopathology,
>Queen's Medical Centre,
>Nottingham NG7 2UH,
>England UK
>
>____________________________________________________________________
>Neil Hand
>Department Histopathology, University Hospital, Nottingham NG7 2UH.
>work : Tel: (0115) 924 9924 extension 43725
>____________________________________________________________________
>
>
>
>
>



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